Product Info Summary
SKU: | M03989 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Pig, Turkey, Yeast, Arabidopsis, Eisenia, Paramecium, Nicotiana |
Host: | Mouse |
Application: | Flow Cytometry, IP, IHC-P, ICC, WB |
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Product info
Product Name
Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody
View all alpha Tubulin Antibodies
SKU/Catalog Number
M03989
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody (Catalog# M03989). Tested in WB, IHC-P, ICC, IP, Flow Cytometry application(s). This antibody reacts with Mouse, Pig, Human, Turkey, Eisenia, Paramecium, Nicotiana, Yeast, Arabidopsis.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03989)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
TU-01
Isotype
Mouse IgG1
Immunogen
Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization. The antibody TU-01 recognizes a defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M03989 is reactive to TUBA1A in Human, Mouse, Pig, Turkey, Yeast, Arabidopsis, Eisenia, Paramecium, Nicotiana
Applications
M03989 is guaranteed for Flow Cytometry, IP, IHC-P, ICC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
50.136kDa
Background of alpha Tubulin
The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –; this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml, intracellular staining.
Western blotting: 1-2 μg/ml, reducing conditions.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Immunocytochemistry staining of TUBA1A using Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody (M03989).
Immunocytochemistry staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01; green) and anti-Vimentin (VI-01; cat. no. 11-254-C100; red). Nucleus is stained with DAPI (blue).Click image to see more details
Figure 2. Immunocytochemistry staining of TUBA1A using Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody (M03989).
Immunocytochemistry staining of HeLa human cervix carcinoma cell line using anti-alpha-tubulin (TU-01; red). Nucleus is stained with DAPI (blue).Click image to see more details
Figure 3. Immunocytochemistry staining of TUBA1A using Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody (M03989).
Immunocytochemistry staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01; green). Nucleus is stained with DAPI (blue).Click image to see more details
Figure 4. Immunohistochemistry validation of TUBA1A using Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody (M03989).
Immunohistochemistry staining of human heart (paraffin sections) using anti-alpha-tubulin (TU-01).Commercially tested by LifeSpan BioSciences.
For more protocol information of IHC
Click image to see more details
Figure 5. IP validation of TUBA1A using Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody (M03989).
Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of alpha tubulin is around 50 kDa.L = lysate
IPr = immunoprecipitate (reducing conditions)
IPn = immunoprecipitate (non-reducing conditions)
Click image to see more details
Figure 6. Western blotting validation for Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody M03989
Use of anti-alpha-tubulin antibody TU-01 as a loading control (A) in an Western blotting experiment revealing the staining pattern of various cell lysates by a newly developed monoclonal antibody (B).Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For TUBA1A (Source: Uniprot.org, NCBI)
Gene Name
TUBA1A
Full Name
Tubulin alpha-1A chain
Weight
50.136kDa
Superfamily
tubulin family
Alternative Names
alpha tubulin loading control; alpha tubulin monoclonal; alpha Tubulin; Alpha-Tubulin 3; B-ALPHA-1; DM1A tubulin; dm1a monoclonal; DM1a tubulin; DM1A; FLJ25113; hum-a-tub1; hum-a-tub2; LIS3; TUBA1 monoclonal; TUBA1A; TUBA3; tubulin alpha-1A chain; Tubulin alpha-3 chain; Tubulin B-Alpha-1; tubulin monoclonal; tubulin, alpha 1a; tubulin, alpha 3; tubulin, alpha, brain-specific TUBA1A B-ALPHA-1, LIS3, TUBA3 tubulin alpha 1a tubulin alpha-1A chain|hum-a-tub1|hum-a-tub2|tubulin B-alpha-1|tubulin alpha-3 chain|tubulin, alpha, brain-specific
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TUBA1A, check out the TUBA1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TUBA1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-alpha-Tubulin Purified TUBA1A Monoclonal Antibody (M03989)
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No publications found for M03989
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